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Microarray Technology: Methods And Applications PDF Description:
The microarray method has been a widely applied technology. There is a considerable amount of technical and scientific literature published recently. This book aims to provide updates of this established technology on topics such as methods and applications, as well as advances in applications of the microarray method to assay substances such as proteins, glycans, and whole cells. The 19 chapters, which recognized experts write on each topic, including reviews, detailed protocols, and an outlook of the nucleic acid microarrays and the cell/tissue microarrays.
This volume provides updates on this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs, proteins, glycans, and whole cells.
Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microarray Technology: Methods and Applications aims to ensure successful results in the further study of this vital field.
Content of oligoarray2_1.tar.gz
PART I INTRODUCTION
1 Overview of Microarray Technology
PART II ADVANCES ON TECHNIQUES IN MICROARRAY TECHNOLOGY
2 Surface Functionalization for Immobilization of Probes on
3 The “Clickable” Method for Oligonucleotide Immobilization
Onto Azide-Functionalized Microarrays
4 Microarray Developed on Plastic Substrates
5 Detection and Quantification of MicroRNAs by Ligase- Assisted
Sandwich Hybridization on a Microarray
6 Probe Design Strategies for Oligonucleotide Microarrays
PART III APPLICATIONS OF MICROARRAY TECHNOLOGY TO NUCLEIC ACID ASSAYS
7 Analyzing Illumina Gene Expression Microarray Data Obtained
From Human Whole Blood Cell and Blood Monocyte Samples
8 Microarray Technology and Its Applications for Detecting Plasma
microRNA Biomarkers in Digestive Tract Cancers
9 Application of DNA Microarray to Clinical Diagnostics
10 High-Throughput DNA Array for SNP Detection of KRAS Gene
Using a Centrifugal Microfluidic Device
11 Single Nucleotide Polymorphisms as Genomic Markers
for High-Throughput Pharmacogenomic Studies
12 DNA Microarray-Based Diagnostics
PART IV APPLICATIONS OF MICROARRAY TECHNOLOGY TO OTHER BIOLOGICAL ASSAYS
13 High-Throughput Screening of Substrate Specificity for Protein
Tyrosine Phosphatases (PTPs) on Phosphopeptide Microarrays
14 Nanotechnology in the Fabrication of Protein Microarrays
15 Epitope Mapping Using Peptide Microarray in Autoantibody Profiling
16 Preparation of Glycan Arrays Using Pyridylaminated Glycans
17 Competitive Immunoassays Using Antigen Microarrays
18 Parallel Syntheses of Peptides on Teflon-Patterned Paper
19 Cell Microarrays for Biomedical Applications